On your travels through remote Australia your colleague was introduced by the locals to a plant that was said to have “healing properties”. You have obtained samples of the plant and prepared extracts from various tissues. One extract from the bark of the tree is of particular interest to your research group. Initial tests reveal that the compound appears to have an inhibitory effect on cell proliferation. In this practical session you will measure how well a potential anticancer agent (Test Drug A) inhibits the proliferation (growth) of cancer cells lines in culture. You will be provided with two flasks, one untreated control and one that has been treated with 50 nM Test Drug A for 72 hours. Each pair will receive one cell line. We will collate the class data in order to compare the effect of Test Drug A on different cell lines. These practical sessions will introduce you to techniques that are widely used in the study of cells, and beyond the basic staining methods you have used in first year biology. The overall aim of this practical is to determine how effective the anticancer drug is at preventing the growth of different types of cancer cells in culture
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5627931_1_sle206-manual-t2-2020.pdf
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