Both the AAS and AES should already be setup and ready to go. Similarly, the AAS and AES software…

Both the AAS and AES should already be setup and ready to go. Similarly, the AAS
and AES software should already be loaded on the computer and all necessary operating
parameters for the experiment defined. However, it is wise and a good practice to
double-check them. To check the instrument parameters for AAS, proceed as follows:
(a) Click with the right mouse button on the Method icon (on the left-hand toolbar)
and select Properties. Confirm the System Type is set to Flame.
20 40 60 80 100 300
1.4
1.2
1.0
0.8
0.6
0.4
0.2
Absorbance
4.8 Å
2.4 Å
1.2 Å
0.6 Å
0.3 Å
Fe, µg mL-1
Experiment 3 Atomic Spectrometry 3-12
(b) Select the Description tab on the Method notebook and confirm that the atomic
symbol for iron is the element being analysed. If it is not, enter the atomic symbol
for iron in the Element Field.
(c) Next, confirm the operating parameter for the lamp by choosing the Instrument tab
of the method notebook. The parameters should appear as in Table 1. If not, adjust
them appropriately.
Table 1. Summary of Instrument Parameters for Fe analysis.
Lamp current: 7 mA
Wavelength 248.3 nm
Slit width 0.2 nm
Slit height normal
Background correction: off.
(d) Finally, check the initial optimisation on the hollow cathode lamp and burner as
follows:
(i) Select the Instrument icon (on the left-hand toolbar) and a picture of the
instrument should appear. Double click the “bulge” on the top left of the
picture of the instrument. Adjust the vertical and horizontal alignment
knobs (see Figure 8 for their position) to achieve a maximum signal on the
reference HC meter, i.e. maximum light throughput (ensure that the burner
is not in the light path). If the needle in the meter goes over the range, press
the spacebar. Take care not to unscrew the knobs completely. Close the
status panel by clicking the x in the top right of the status panel window.
(ii) Place a white card on top and orthogonally and centrally along the burner.
Using the vertical adjustment knob raise the burner until the centre of the
light beam is 6-8 mm above the burner. Use the horizontal adjustment knob
and rotation control to place the centre of the light beam above the burner
slot and the beam parallel to the slot. A final optimisation of the burner
position will be performed later whilst aspirating a standard.
Attachments:
3067577_1_Results-Data-from-Experiment.docx
3067577_2_Sample-Report-of-Previous-student.docx
3067577_3_Experiment-2-File-Important.pdf
3067577_4_Copy-of-regress.xls